(also known as a transposon)
A DNA sequence that can move from one section of the genome to another, affecting chromosome shape and structure as well as gene expression.
- Cut-and-paste transposons move by excision from their original location and insertion into a new location, catalyzed by a transposase enzyme encoded by the transposon itself.
- Replicative transposons are replicated and the copy is inserted at a new location without loss of the transposon at the original location; this is also catalyzed by a transposase enzyme encoded by the transposon.
- Retrotransposons move via RNA transcription of the transposon followed by reverse transcription of the RNA molecule and insertion of the newly synthesized DNA.
Identifying transposon insertions and their effects from RNA sequencing data.
Julian R. de Ruiter, Sjors M. Kas, Eva Schut, David J. Adams, Marco J. Koudijs, Lodewyk F.A. Wessels, Jos Jonkers.
Nucleic Acids Research gkx461. DOI https://doi-org.ezproxy1.lib.asu.edu/10.1093/nar/gkx461. Link to Article
Continue reading “RNA-sequencing for Transposon Identification”
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