Posted in Sequencing Technology and Methods

Stranded mRNA Sequencing

mRNA – messenger RNA – is typically around 5% of the total RNA in a cell, and in most eukaryotic organisms the vast majority of it is tagged with a poly(A) tail. This allows these molecules to be isolated out from a total RNA sample, reducing the number of reads needed to sequence the transcriptome or analyze gene expression. Continue reading “Stranded mRNA Sequencing”