Identifying transposon insertions and their effects from RNA sequencing data.
Julian R. de Ruiter, Sjors M. Kas, Eva Schut, David J. Adams, Marco J. Koudijs, Lodewyk F.A. Wessels, Jos Jonkers.
Nucleic Acids Research gkx461. DOI https://doi-org.ezproxy1.lib.asu.edu/10.1093/nar/gkx461. Link to Article
Continue reading “RNA-sequencing for Transposon Identification”
It’s always useful to have a method for determining the relative quality of one’s source material. If you’re starting with garbage, you’re unlikely to have anything but garbage at the end, no matter how well you execute your protocols or how skillfully you massage your data. For RNA, one of the primary means of determining quality is the RNA Integrity Number, or RIN score, developed by Agilent. Continue reading “What is a RIN score?”
The composition of RNA in a cell can be roughly estimated at 80% ribosomal RNA, 15% transfer RNA, and 5% messenger RNA. With total RNA sequencing, all of those subtypes (as well as any small RNAs) are converted into cDNA for sequencing. Continue reading “Total RNA Sequencing”