Posted in Literature

RNA-sequencing for Transposon Identification

Identifying transposon insertions and their effects from RNA sequencing data.

Julian R. de Ruiter, Sjors M. Kas, Eva Schut, David J. Adams, Marco J. Koudijs, Lodewyk F.A. Wessels, Jos Jonkers.

Nucleic Acids Research gkx461. DOI Link to Article

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Posted in Sequencing Technology and Methods

What is a RIN score?

It’s always useful to have a method for determining the relative quality of one’s source material. If you’re starting with garbage, you’re unlikely to have anything but garbage at the end, no matter how well you execute your protocols or how skillfully you massage your data. For RNA, one of the primary means of determining quality is the RNA Integrity Number, or RIN score, developed by Agilent. Continue reading “What is a RIN score?”